Skip to main content
. 2003 Oct;77(19):10260–10269. doi: 10.1128/JVI.77.19.10260-10269.2003

FIG. 1.

FIG. 1.

Schematic of recombinant viruses. (A) Interspecies chimeric virus fMHV-JHM B3b. fMHV-JHM carries the region from gene 1 through the 5′ end of HE from JHM.IA, the 3′ end of HE from MHV-A59, the ectodomain of FIPV gene 3, the transmembrane domain and endodomain of gene 3 from MHV-A59, and the region from gene 4 through the 3′ untranslated region (3′ UTR) from MHV-A59 (45). This chimeric virus infects only feline cells. (B) RNA transcribed from pJHM. As an example, construction of rJHM.IA is shown. Donor RNA (8 kb) was transcribed in vitro as described in Materials and Methods. pJHM.IA carries sequences entirely derived from JHM.IA. (C) Construction of rJHM.IA. fMHV-JHM B3b-infected feline cells were transfected with donor RNA transcribed from pJHM.IA to create rJHM.IA. (D) Donor RNA from pJHM.SD was used to create rJHM.SD. Point mutations were introduced into pJHM.IA to create the recombinant viruses listed. The sources of genomic or donor RNA segments are indicated by open rectangles for JHM.IA, hatched rectangles for FIPV, and shaded rectangles for MHV-A59. S1-S2 cleavage occurs between nt 2307 and 2308.