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Thermostability of spike variants. Cell-free virus was incubated in solutions at a pH of 6.0 (A), 7.0 (B), or 8.0 (C) at 37°C. Aliquots were removed at the indicated time points and titered on HeLa-MHVR cells. The fraction of virus surviving at each time point is shown. Symbols: (□), JHM-IA; ○, JHM-SD; ▪, rJHM.IA; •, rJHM.SD; +, rJIA-S.S310G. Data are representative of four independent experiments.
