FIG. 2.
Partial 96-well dot blot showing the PK-resistant PrP signal visualized with primary antibody 6B10. Signals from untreated control (Cont) cells and curcumin-inhibited (Cur) cells are indicated. The latter were incubated in the presence of 10 μM curcumin, a known inhibitor of PrPSc in RML-infected cells (6). Other dots represent signals from ScN2a cells after incubation with 10 μM concentrations of various compounds. Some of these spots have an intensity comparable to that of controls, indicating no inhibition of PrPSc formation. Others that are less intense were due to compounds with various inhibitory strengths or toxicities.