FIG. 3.

Intracellular localization of CPV capsids microinjected into cytoplasm along with antibodies against the motor protein dynein or kinesin. The cells were incubated for 6 h after injection, after which they were fixed and stained for capsids with rabbit polyclonal anti-capsid IgG followed by Alexa-546-conjugated goat anti-rabbit IgG (red). The presence of the injected anti-dynein MAb, the anti-kinesin MAb, and control mouse IgG was detected using Alexa-488-conjugated goat anti-mouse IgG (green).