FIG. 4.

Lassa virus wild-type Z protein and mutants. (A) The amino acid sequence of Lassa virus Z protein, strain Josiah, is shown in one-letter code (NCBI accession number NC_004296/004297). Amino acids associated with particular structures and possible functions are shown in boldfaced single letters. The cysteine and histidine residues that are proposed to form the RING domain are underlined, the two tetrapeptides postulated as late domains are doubly underlined, and the hydrophobic peptide (aa 50 to 61) is indicated with a dotted line. (B) Wild-type Z, deletion, and substitution mutants used in this study are shown schematically.