FIG. 3.

VSVG(HA)GFP(NA) virus-infected cells express VSVG and GFP. MDCK cells were infected with VSVG(HA)GFP(NA) virus or WSN virus and overlaid with 1.0% agarose. The infected cells were incubated for 48 h at 37°C, and representative plaques were photographed under normal light (A and B) and under fluorescent light (C and D). The cells were fixed and treated with 0.1% Triton X-100 in 3% formaldehyde solution. The viral proteins were detected by immunostaining with anti-WSN HA monoclonal antibody (E and F), anti-VSVG monoclonal antibody (G and H), or anti-WSN NP monoclonal antibody (I and J) as the primary antibody and biotinylated secondary antibody by using the Vectastain ABC kit.