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Incorporation of the VSVG protein into VSVG(HA)GFP(NA) virus. Concentrated WSN and VSVG(HA)GFP(NA) viruses, and VSV were lysed in a sample buffer. Viral proteins were treated with 2-mercaptoethanol, separated by SDS-10% polyacrylamide gel electrophoresis, transferred to a polyvinylidene difluoride membrane, and incubated with anti-VSVG monoclonal antibody or anti-WSN HA monoclonal antibody.
