FIG. 7.

EBNA3AHT inactivation has minimal effect on latent EBV gene expression. LCL 41-13 cells infected with EBV expressing recombinant EBNA3AHT were washed and cultured with (+4HT) or without (−4HT) 4HT for indicated numbers of days. Protein lysates were prepared from EBNA3AHT-infected LCL cells (E3AHT LCLs) or from control LCL 1c12 cells (wt LCL), and Western blot analyses used EBV-immune human serum to detect EBNA3AHT (A), EBNA3B (B), and EBNA2 and EBNA1 (C) and used the S12 monoclonal antibody to detect LMP1 (D).