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. 2003 Oct;77(19):10566–10574. doi: 10.1128/JVI.77.19.10566-10574.2003

FIG. 1.

FIG. 1.

GST-16E7 activates purified cyclin E/CDK2 and cyclin A/CDK2 complexes. Autoradiographs demonstrate phosphorylation of histone H1 and Rb (pRb) by CDK2/cyclin E (A) and CDK2/cyclin A (B). (A) E7 potently upregulates CDK2/cyclin E histone H1 kinase activity while having modest effects on Rb kinase activity. Addition of p27 eliminates this effect and reduces activity to below baseline levels. (B) CDK2/cyclin A histone kinase activity is also dramatically upregulated by E7, and the effect is negated by addition of p27.