TABLE 2.
Infection with wild-type and variant MMTV pseudovirusesa
| Pseudovirus | Avg LFU/ml ± SD
|
||||
|---|---|---|---|---|---|
| MCF-7 | MCF-7/vp5 | T47D | 293T | NMuMG (10−4) | |
| pENVC3H | 0 | 0 | 17.3 ± 5.5 | 49.9 ± 49.0 | 1.56 ± 0.11 |
| pENVC3H + Black 6-5D | ND | ND | 4.0 ± 6.9 | 106.7 ± 92.4 | 0.19 ± 0.05 |
| Tyr40 | 0 | 0 | 0 | 0 | 0.41 ± 0.06 |
| Ser40 | 0 | 0 | 0 | 0 | 0 |
| Ala40 | 0 | 0 | 0 | 0 | 0 |
| Glu42 | 0 | 0 | 0 | 0 | 1.28 ± 0.11 |
| pENVRIII | 0 | 0 | 34.0 ± 19.9 | 120.7 ± 108.0 | 0.43 ± 0.06 |
| pENVRIII + Black 6-5D | ND | ND | 60.0 ± 36.0 | 210.7 ± 164.8 | 0.06 ± 0.03 |
| pENVRIIIM | 2, 0 | 12, 0 | 60.8 ± 24.2 | 142.3 ± 173.4 | 1.04 ± 0.13 |
| pENVRIIIM + Black 6-5D | ND | ND | 64 ± 30.2 | ND | 0.16 ± 0.05 |
| No Env | ND | ND | 13.75 ± 4.9 | 34.17 ± 22.7 | 0 |
a
Data are presented as the averages for supernatant from four to fifteen experiments ± standard deviation with the exception of the RIIIM infection of MCF-7 and MCF-7/vp5 cells, which was performed twice. All pseudoviruses were checked by Western blot analysis (i.e., see Fig. 3) to ensure that equal amounts of viral proteins were produced. ND, not determined.