Figure 3.

Basal telomere pattern is similar in ter1-Kpn and ter1-Kpn;rap1-ΔC strains. Telomeric patterns of EcoRI + KpnI-digested genomic DNAs from two clones of the double mutant strains ter1-Kpn;rap1-ΔC (KpnΔc1 and KpnΔc2) and a single ter1-Kpn strain (Kpn). The strains are the same clonal lines shown in Fig. 2. The sizes of the telomeric restriction fragments (bands between 1- and 4-kb markers) reflect the lengths of the remaining basal internal tracts of wild-type repeats, which are not cleaved by KpnI restriction enzyme. The diffuse hybridization signal below the 0.5-kb marker is from the Kpn mutant repeat DNA cleaved off the telomeres.