Multiprobe RNase protection assay for various cytokines on RNA from antigen-activated TGF-β1-transduced and untransduced MBP-specific T cells. Scanning of the band densities, in comparison with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as control, showed a moderate relative increase in TGF-β1 mRNA in the transduced cells. There was also a slight increase in LT-β, and no change in LT-α, TNF-α, IFN-γ, or MIF (migration inhibitory factor).