Figure 4.
15N-1H HSQC-TROSY spectrum of the full-length 97-kD IF2-1 from E. coli. The spectrum was obtained at 20°C with a proton resonance frequency of 500 MHz, in 10 mM phosphate buffer (pH 6), 100 mM NaCl, and 1 mM MgCl2. The most well-resolved peaks that have been assigned are labeled. Note that almost all well-resolved peaks arise from Domain I.