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. 2004 Jan;13(1):145–154. doi: 10.1110/ps.03352504

Table 2.

HP-SEC analysis showing binding of wild-type DPPIV and DPPIV mutants to ADA

DPPIV mutant tretention no ADA tretention + ADA Δ(tretention no ADA − tretention + ADA)
Proteins expressed in Sf9 cells
    Wild-type 8.99 8.54 0.45
    N85A 9.12 8.51 0.61
    N92A 9.08 8.60 0.48
    N150A 8.94 8.52 0.42
    N219A 9.09 8.50 0.59
    N229A 8.98 8.49 0.49
    N281A 8.97 8.49 0.48
    N321A 9.00 8.49 0.51
    N520A 9.01 8.50 0.51
    N685A 8.95 8.48 0.47
Proteins expressed in Hi5 cells
    Wild-type 8.85 8.42 0.43
    N85A 8.89 8.45 0.44
    N92A 8.87 8.43 0.44
    N150A 8.83 8.41 0.42
    N219A 8.90 8.47 0.43
    N229A 8.85 8.40 0.45
    N281A 8.87 8.41 0.46
    N321A 8.91 8.42 0.49
    N520A 8.86 8.41 0.45
    N685A 8.79 8.40 0.39