Figure 2.

Alignment of the eight subsets on the basis of structure-based sequence alignment of the four His-Me endonuclease structures. The complete alignment is available as Supplemental Material. The subsets are marked in the following order: subset2, subset8, subset1, subset5, subset3, subset6, subset7, and subset4. (*) The 13 residues used for the final superposition. The alignment was generated using a structure-based sequence alignment obtained from the Biosym software and was curated manually to include the subset sequences.