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Chemical shift deviations from random-coil values for 13Cα (A), 13Cβ (B), 13C′ (C), and 1Hα (DA) resonances of Ala 14 in 50 mM sodium phosphate (pH 5.8) at −5°C (solid circles) and 40°C (open circles). The positive values of 13Cα and 13C′ and negative values of 1Hα are indicators of α-helical structure.
