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Equilibrium unfolding measured by tryptophan fluorescence for the six double mutants V5A/C112S (○), I7V/C112S (▴), V31A/C112S (▾), L33A/C112S (□), L50A/C112S (•), T52S/C112S (▪). C112S (---) is shown as reference. The tryptophan was excited at 295 nm and emission detected at 309 nm. Parameters from the fit (shown as solid lines in the figure) are presented in Table 1.
