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Chevron plots for the six double mutants V5A/C112S (○), I7V/C112S (▴), V31A/C112S (▾), L33A/C112S (□), L50A/C112S (•), T52S/C112S (▪). Shown as reference is the fit of C112S (–––) (raw data not shown). The solid lines are the least-squares fitting of equation 1 to the data.
