Table 2.
Kinetic parameters for folding and unfolding of the azurin mutants in GdmCl
| C112S | V5A/C112S | I7V/C112S | V31A/C112S | L33A/C112S | L50A/C112S | T52S/C112S | |
| kfH2O (s−1) | 62.9 ± 8 | 36.7 ± 4 | 53.8 ± 5 | 8.20 ± 1 | 19.1 ± 4 | 23.5 ± 4 | 59.2 ± 6 |
| kuH2O (s−1) | (5.68 ± 4) • 10−5 | (2.96 ± 2) • 10−5 | (6.56 ± 4) • 10−5 | (7.04 ± 4) • 10−5 | (8.29 ± 2) • 10−3 | (4.22 ± 2) • 10−4 | (6.90 ± 2) • 10−4 |
| mf (M−1) | −1.94 ± 0.3 − (0.904 ± 0.2) [D] | −2.39 ± 0.3 − (0.858 ± 0.2) [D] | −2.67 ± 0.2 − (0.744 ± 0.2) [D] | −4.36 ± 0.5 − (0.209 ± 0.5) [D] | −2.67 ± 1 − (2.21 ± 1) [D] | −3.62 ± 0.6 − (2.22 ± 0.5) [D] | −1.60 ± 0.3 − (1.32 ± 0.2) [D] |
| mu (M−1) | 6.97 ± 0.6 − (0.871 ± 0.1) [D] | 8.97 ± 0.7 − (1.43 ± 0.2) [D] | 6.71 ± 0.6 − (0.839 ± 0.1) [D] | 9.20 ± 0.7 − (1.72 ± 0.2) [D] | 6.29 ± 0.6 − (0.997 ± 0.2) [D] | 6.06 ± 0.7 − (0.922 ± 0.2) [D] | 5.78 ± 0.5 − (0.706 ± 0.1) [D] |
| Δ GU−Nkin (kJ mole−1) | 33.9 ± 2 | 34.3 ± 2 | 33.2 ± 2 | 28.4 ± 1 | 18.8 ± 1 | 26.2 ± 1 | 27.7 ± 1 |
| Φf (H2O) | — | — | — | 0.53 | 0.26 | 0.32 | — |
| Φf (0.5 M GdmCl) | — | — | — | 0.63 | 0.33 | 0.50 | — |
| βTS | 0.53 | 0.47 | 0.52 | 0.44 | 0.59 | 0.65 | 0.58 |