Figure 5.
The distribution of candidate fragments for engineering of native ubiquitin. The distribution of candidate fragments is projected in two coordinates, sequence identity, and hydrophobicity scale difference when their Cα-RNSDs are smaller than 2.5 Å (2.8 Å for BB-III). (A) The candidate fragment distribution for building block-I, (B) for building block-II, and (C) for building block-III of native ubiquitin. Fragments with lower sequence identity as well as the smaller hydrophobicity scale difference are used for engineering ubiquitin.