Table 3.

ΔΔG_assocvalues (kcal/mole) of GpA and MS1 mutants in micelles

Glycophorin A dimer			MS1 trimer
Mutant	ΔΔGexpa	ΔΔGclc	Mutant	ΔΔGexpb	ΔΔGclc
L75A	1.3	0.9	N14V/V7N	−0.6	−0.8
I76A	1.8	1.1	N14Q	−1.8	−0.6
I77A	0.1	0.1	N14E	0.0	0.4
F78A	−0.1	0.0	N14L	>1.8	4.2
V80A	0.4	0.7	N14V	>2.1	4.2
M81A	−0.2	0.0	N14T	>2.4	4.5
V84A	1.0	0.5	N14S	>2.4	6.6
I85A	−0.4	0.0	N14A	>3.0	6.8
G86A	−0.1	−0.1
T87A	0.9	1.5

ΔΔG_assoc = ΔΔG_assoc (mutant) − ΔΔG_assoc (wild type); the values are negative for stabilizing replacements.

^a Data of Fleming and Engelman (2001a).

^b Based on data of Gratkowski et al. (2001) and Lear et al. (2003). N14D mutant was not included, because it forms a mixture of monomers, trimers, and hexamers (Gratkowski et al. 2001).