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Fraction of the unfolded state as a function of GuHCl denaturant, as determined from isothermal equilibrium denaturation data monitored by CD, for wild-type 2,5-DKGRA (filled circles) and the F22Y/K232G/R238H/A272G 2,5-DKGRA mutant (open circles). The solid line shows the fit to the two-state model in each case.
