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Self-assembly in detergent solution as analyzed by SDS-PAGE analysis. Proteins overexpressed in TOP10 cells were solubilized, electrophoresed at equivalent starting concentrations in sample buffer, and visualized by Western blotting. (Left) Electrophoresis of the leu20 protein not boiled in SDS sample buffer. (Right) leu20 was monomeric upon sample boiling, whereas its asparagine mutants still formed various oligomeric species when asparagine was located between positions 8 and 15.
