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HPLC ESI mass spectra of α-crystallins labeled with deuterium under different conditions. (A) No exposure to D2O. (B) Exposed to D2O at 37°C for 112 sec. (C) Completely exchanged in 95% D2O. αA-S and αB + P refer to αA that has lost the C-terminal Ser and phosphorylated αB, respectively.
