Figure 2.

GdmCl-induced denaturation measured at 20°C for 53BP1-BRCT, together with that measured previously for BRCA1-BRCT (Ekblad et al. 2002) for comparison. The buffer used was 50 mM sodium phosphate (pH 8.0), 500 mM NaCl, and 5 mM DTT. The protein concentration was 0.6 μM, and the excitation wavelength was 280 nm. Data are plotted as the average emission wavelength (see Ekblad et al. 2002), and the dotted lines are the fits to a three-state model.