Figure 2.

Multiangle light scattering (MALLS) and dynamic light scattering (DLS) on soluble-state SC3. (A) Elution of soluble-state SC3 from a size-exclusion chromatography (SEC) column, which was subsequently detected in-line by a MALLS detector (solid line) and a refractive index detector (dotted line). (B) DLS analysis of soluble-state SC3: soluble-state SC3 in 50 mM sodium phosphate (pH 7.0) before SEC (left) and soluble-state SC3 in 50 mM sodium phosphate (pH 7.0) after SEC (right). Peak P₂ was collected and measured immediately.