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. 2004 Mar;13(3):810–821. doi: 10.1110/ps.03367304

Figure 4.

Figure 4.

Oligomerization of SC3 under various buffer conditions. (A) The same experiment as Fig. 1 ▶, but pure water was used instead of 50 mM sodium phosphate (pH 7.0) to suspend TFA-treated lyophilized SC3 and to equilibrate the column. The pH of the sample and the column effluent was about 6. (B) The same experiment as Fig. 1 ▶, but 50 mM sodium chloride in water (pH ~6), was used instead of 50 mM sodium phosphate (pH 7.0) to suspend SC3 and equilibrate the column. (C) The same experiment as Fig. 1 ▶, but 50 mM sodium bicarbonate (pH 9.0), was used instead of 50 mM sodium phosphate (pH 7.0) to suspend SC3 and equilibrate the column.