Table 1.

Yield of soluble protein for each of the Ub-fusion proteins expressed from pHUE

Purified protein	Size (kD)a	Structureb	Yield (mg/L)c
His6Ub–peptide	14.1	Polypeptide	14.30
His6Ub–T-ag-NLS	13.7	Polypeptide	13.30
His6Ub–Ova	14.5	Polypeptide	17.55
His6Ub–SUMO	24.5	Monomer	22.83
His6Ub–M-GSTP1	34.0	Dimer	25.56
His6Ub–P-GSTP1	33.9	Dimer	26.85
His6Ub–GSH-S	63.1	Dimer	3.58
His6Ub–β-gal	129.2	Tetramer	3.30

a Size of intact fusion protein. His₆Ub portion contributes 10.7 kD.

^b Expected subunit composition in solution.

^c Yield given as purified soluble protein in milligrams per liter of Escherichia coli culture.