Figure 3.

¹H-NMR spectra of cpBPTI variants. Spectra were collected at a proton resonance frequency of 600 MHz, and at pH 4.7 and room temperature as described previously (Zhang and Goldenberg 1997a), and the spectrum for the wild-type protein is taken from that publication. The well-resolved amide proton resonances and that of the ɛ proton of Tyr23 were identified from the published assignments (Wagner et al. 1987a,b).