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Relative contact order (RCO) calculated for wild-type BPTI and for each of the 57 possible circularly permuted variants. In A, the curve represents the RCO calculated for all types of interactions, as described by Plaxco et al. (1998), with the filled circles identifying the wild-type sequence and the four variants examined in this study. In B, the values of RCO were calculated from only the disulfide bonds.
