Table 2.

Stability changes of native BPTI and folding intermediates, relative to circular BPTI

Variant	I (kcal/mole)	II (kcal/mole)	II′ (kcal/mole)	N (kcal/mole)
Wild-type	−0.1	0.9	−0.4	−1.2
cp16	−0.2	1.1	0.8	3.4
cp27	0.0	0.8	4.9	5.0
cp41	0.1	0.7	1.4	4.2
cp47	0.2	1.5	3.8	7.8

Calculated using equation 1 and the rate constants of Table 1. A positive value indicates that the indicated linear form is less stable than the corresponding form of circular BPTI. For I, the equilibrium constants were calculated as K = k₁/k₋₁; for II, K = (k₁k₂)/(k₋₁k₋₂); for II′, K = (k₁k₂k_rearr)/(k₋₁k₋₂k_−rearr); for N, K = (k₁k₂k_rearrk₃)/(k₋₁k₋₂k_−rearrk₋₃).