Table 3.
Calculated effective concentrations of chain termini
| Variant | Ceff,II′ | Ceff,N |
| Wild type | 3.7 × 10−3 | 1.0 × 10−3 |
| cp16 | 3.0 × 10−2 | 2.5 |
| cp27 | 2.9 × 101 | 3.6 × 101 |
| cp41 | 7.6 × 10−2 | 9.5 |
| cp46 | 4.6 | 3.9 × 103 |
Ceff,N for the cp16 variant was calculated from equation 3, using the published equilibrium constants for enzymatic hydrolysis of the Lys15–Ala16 peptide bond of wild-type BPTI (Estell et al. 1980; Siekmann et al. 1988) and of a dipeptide (Homandberg et al. 1978; Deschereverl et al. 2003). The other values in the table were calculated from equation 5 and the destabilizations listed in Table 2, assuming Ceff,U = 7.5 mM.