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. Author manuscript; available in PMC: 2008 Apr 3.
Published in final edited form as: J Leukoc Biol. 2007 Jun 12;82(3):465–476. doi: 10.1189/jlb.1106678

Fig. 4.

Fig. 4

Slit-2 inhibits the CXCL12-induced chemotaxis of primary monocytes and CD4+ T cells. Monocytes (A) and CD4+ T cells (B) were isolated from peripheral blood by using negative selection kits according to the manufacturers’ instructions (Dynal Biotech and StemCell Technologies). The cells were pretreated with Slit-2 supernatant (100 μg/ml) or control supernatant (100 μg/ml) and then subjected to chemotactic assays in the presence of CXCL12 (50 ng/ml), as described in Materials and Methods. The experiments were done in triplicate and are presented as the mean ± SE. The data are representative of four different experiments. *, P < 0.05.