Table 2.
Mips codea | Whole arrayb | selected | FB | EFD | MD | R | EFD1 | EFD2 | R1 | R2 | R3 | |
Metabolism | 1 | 16.1 | 20.3 | 21.5 | 20.1 | 17.2 | 20.9 | 18.3 | 21.1 | 21.7 | 25.4 | 18.4 |
Energy | 2 | 2.9 | 3.4 | 3.0 | 2.8 | 2.1 | 4.5 | 2.2 | 3.1 | 3.0 | 5.0 | 4.4 |
Cell Cycle and DNA processing | 3 | 2.9 | 2.5 | 1.8 | 3.4 | 1.4 | 1.9 | 3.3 | 3.5 | 0.7 | 1.9 | 2.4 |
Transcription | 4 | 5.2 | 4.1 | 4.3 | 4.1 | 4.1 | 3.9 | 4.4 | 4.0 | 3.3 | 3.1 | 4.6 |
Protein synthesis | 5 | 2.0 | 1.7 | 1.5 | 1.6 | 1.8 | 2.0 | 1.5 | 1.6 | 2.7 | 0.7 | 2.6 |
Protein fate | 6 | 6.6 | 5.4 | 4.6 | 5.0 | 5.5 | 6.0 | 4.5 | 5.3 | 4.0 | 4.7 | 7.1 |
Cellular transport & mechanisms | 8 | 2.4 | 1.7 | 1.8 | 1.6 | 2.6 | 1.7 | 2.1 | 1.3 | 0.7 | 1.7 | 1.8 |
Cellular comm/signaling | 10 | 6.4 | 5.6 | 6.5 | 5.5 | 5.1 | 5.6 | 5.9 | 5.4 | 9.0 | 5.6 | 4.9 |
Cell rescue, defense & virulence | 11 | 3.6 | 4.0 | 4.1 | 4.1 | 4.6 | 3.6 | 3.3 | 4.6 | 5.7 | 4.3 | 2.9 |
Regulation of/interaction with cellular environment | 13 | 1.7 | 1.6 | 2.1 | 1.7 | 2.8 | 1.1 | 1.7 | 1.7 | 0.3 | 1.1 | 1.3 |
Cell fate | 14 | 3.2 | 2.6 | 2.3 | 2.5 | 1.5 | 3.2 | 2.4 | 2.6 | 3.7 | 2.6 | 3.3 |
Systemic regulation of/interaction with environment | 20 | 1.1 | 1.3 | 1.5 | 1.3 | 1.1 | 1.1 | 1.9 | 0.9 | 1.3 | 1.1 | 1.1 |
Development | 25 | 1.0 | 1.2 | 1.2 | 1.4 | 1.1 | 0.9 | 1.2 | 1.5 | 2.0 | 0.6 | 0.8 |
Transposable elements, viral and plasmid proteins | 29 | 0.1 | 0.1 | 0.1 | 0.0 | 0.0 | 0.1 | 0.0 | 0.1 | 0.0 | 0.2 | 0.0 |
Control of cellular organisation | 30 | 2.7 | 3.2 | 2.7 | 3.8 | 4.6 | 2.4 | 4.8 | 3.3 | 2.7 | 2.3 | 2.4 |
Subcellular localisation | 40 | 19.1 | 18.1 | 15.9 | 17.4 | 19.8 | 19.2 | 17.5 | 17.4 | 16.0 | 18.0 | 20.5 |
Protein activity regulation | 62 | 0.0 | 0.1 | 0.2 | 0.1 | 0.0 | 0.0 | 0.2 | 0.1 | 0.0 | 0.0 | 0.0 |
Protein with binding function or cofactor requirement | 63 | 3.2 | 2.9 | 2.7 | 2.8 | 4.4 | 2.8 | 2.3 | 3.1 | 2.3 | 3.1 | 2.7 |
Storage protein | 65 | 0.1 | 0.0 | 0.1 | 0.0 | 0.0 | 0.0 | 0.1 | 0.0 | 0.0 | 0.1 | 0.0 |
Transport facilitation | 67 | 3.9 | 3.6 | 4.4 | 3.2 | 2.9 | 3.7 | 3.4 | 3.1 | 3.0 | 4.3 | 3.7 |
Unclassified | 98 or 99 | 15.7 | 16.7 | 17.8 | 17.4 | 17.5 | 15.2 | 19.1 | 16.4 | 18.0 | 14.2 | 15.1 |
The table shows the distribution of classifications as a percentage of the total number of classifications.
a Apple genes for each EST on the array were used to identify Arabidopsis homologues using BLAST with a cutoff of 1 e-5. Where a putative homologue was identified, the Arabidopsis MIPS (Munich Information centre for Protein Sequences, funcat version 1.3) classification(s) for that gene were applied to the apple EST.
b For the whole array, for the features selected as changing during fruit development, and for each of the clusters and sub-clusters the frequency of occurrence for each functional category is shown as a percentage of the total number of functional categories for that cluster (or sub-cluster). FB = Full bloom; EFD = early fruit development; MD = mid-development; R = ripening; R1, R2, R3 = the 3 ripening sub-clusters; EFD1, EFD2 = the 2 early fruit development sub-clusters.