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. 2008 Feb 18;7:21. doi: 10.1186/1476-4598-7-21

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Copyright © 2008 Austinat et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of different mutations of β-catenin on the expression of the reporter gene 'TOP-flash'. Cells from the cell-line HuH7 were transfected with the reporter gene 'TOP-flash' harbouring LEF-1/TCF binding sites for β-catenin and the corresponding 'FOP-flash' without these sites. Each of the two reporters was cotransfected with several expression plasmids for different β-catenin forms: The construct 'bCATa' as a control not expressing any functional protein, the vector 'pBatem' expressing a truncated β-catenin, 'pCS2', expressing a form with a mutation of all N-terminal phosphorylation sites to alanine and in addition plasmids with mutations found in human HCCs: H36P, P44A, S45F and S45P. The expression from each co-transfection with 'TOP-flash' was compared to cotransfection with 'FOP-flash' in order to determine the factor of enhancement mediated by the mutants.