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Effect of PGRN/GRN on soma size and neurite outgrowth. (A–D) NF-H staining of motor neuron (A and B) and cortical culture (C and D) treated with GRN E (B), PGRN (D), or control (A and C; Bar, 50 μm). (E and F) Effect of 100 ng/ml GRN E or PGRN (in the absence and presence of 100 ng/ml SLPI) on soma size (E) and maximal neurite length (F; n = 109–476; *, significantly different from control [P < 0.001]; #, significantly different from GRN E [P < 0.03]). Error bars show mean ± SEM.
