Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Apr 7;181(1):79–88. doi: 10.1083/jcb.200709060

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2008, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 3. — Clp1 regulates Cdc15 dynamics at the CR. (A) Representative images from confocal FRAP measurements of Cdc15-GFP at the CR in wild type, clp1Δ, clp1-C286S, and mid1Δ_431-481 cells. B, bleach region; NB, nonbleached region (used to correct for overall bleaching); BKGD, background region (used to correct for overall bleaching). Bar, 5 μm. (B) Fluorescence recovery curves including half-times with standard error and number of cells analyzed per strain. Best-fit curves derived from the mean intensity values of n cells (Prism software) were used to calculate half-times and mobile fractions. R² values: wt, 0.9877; clp1Δ, 0.9802; clp1-C286S, 0.9819; and mid1Δ_431-481, 0.9762. (C) Mobile fraction graphs from confocal FRAP measurements of Cdc15-GFP. Asterisks indicate significant differences between wild-type and experimental cells as determined by student's t test. P-values and standard error are included.