Skip to main content
. 2008 Apr 7;181(1):79–88. doi: 10.1083/jcb.200709060

Table I. Yeast strains used in this study.

Strain Genotype Reference
KGY246 h− ade6-M210 leu1-32 ura4-D18 Laboratory stock
KGY648 h+ cdc3-124 clp1∷ura4 ade6-M21X leu1-32 ura4-D18 Laboratory stock
KGY1278 h+ rlc1-GFP:ura4 ade6-M210 leu1-32 ura4-D18 Laboratory stock
KGY1985 h+ cdc25-22 cdc15-HA:kanR clp1∷ura4 ade6-M210 leu1-32 ura4-D18 This study
KGY2417 h− mid1-GFP:kanR ade6-M210 leu1-32 ura4-D18 Laboratory stock
KGY2882 h− clp1-MYC:kanR ade6-M216 leu1-32 ura4-D18 Trautmann et al. (2001)
KGY3019 h− cdc15-GFP:kanR ade6-M210 leu1-32 ura4-D18 Carnahan and Gould (2003)
KGY3078 h− cdc15-140 mid1-GFP:kanR ade6-M21X leu1-32 ura4-D18 Laboratory stock
KGY3079 h− mid1-GFP:kanR sid4-GFP:kanR ade6-M21X leu1-32 ura4-D18 Laboratory stock
KGY3155 h− cps1-191 mid1-GFP:kanR ade6-M21X leu1-32 ura4-D18 lys1-151 Laboratory stock
KGY3173 h+ nda3-KM311 mid1-GFP:kanR ade6-M210 leu1-32 This study
KGY3350 h− cdc25-22 cdc15-HA:kanR ade6-M21X leu1-32 ura4-D18 This study
KGY3381 h− clp1∷ura4 ade6-M216 leu1-32 ura4-D18 Trautmann et al. (2001)
KGY3382 h− cdc25-22 clp1-GFP:kanR ade6-216 leu1-32 ura4-D18 This study
KGY3388 h+ nda3-KM311 clp1-MYC:kanR leu1-32 This study
KGY3612 h− nda3-KM311 ura4-D18 leu1-32 Laboratory stock
KGY3783 h+ nda3-KM311 clp1∷ura4 ade6-M21X leu1-32 ura4-D18 Wolfe and Gould (2004)
KGY4410 h− nda3-KM311 cdc15-HA:kanR ade6-M21X leu1-32 This study
KGY4630 h+ nda3-KM311 clp1-GFP:kanR leu1-32 ura4-D18 This study
KGY4791 h− cps1-191 clp1∷ura4 ade6-M21X leu1-32 ura4-D18 lys1-151 Trautmann et al. (2001)
KGY4815 h− nda3-KM311 mid1-MYC:ura4 leu1-32 ura4-D18 This study
KGY4833 h+ cdc15-140 clp1∷ura4 ade6-M21X leu1-32 ura4-D18 Laboratory stock
KGY4881 h− clp1-MYC:kanR mid1∷ura4 ade6-M216 leu1-32 ura4-D18 This study
KGY5298 h+ kanR:GFP-myo2 ade6-M210 leu1-32 ura4-D18 Wu et al. (2003)
KGY5318 h+ kanR:YFP-rng2 ade6-M210 leu1-32 ura4-D18 Wu et al. (2003)
KGY5482 h+ cdc25-22 cdc15-HA: kanR mid1∷ura4 ade6-M210 leu1-32 ura4-D18 This study
KGY5594 h− nda3-KM311 cdc15-HA:kanR clp1∷ura4 leu1-32 This study
KGY5598 h+ nda3-KM311 cdc15-HA:kanR mid1∷ura4 ade6-M21X ura4-D18 leu1-32 This study
KGY5615 h− cdc25-22 clp1-GFP:kanR mid1∷ura4 ade6-21X leu1-32 ura4-D18 This study
KGY5616 h− nda3-KM311 clp1-GFP:kanR mid1∷ura4 ade6-M21X leu1-32 ura4-D18 This study
KGY5617 h+ nda3-KM311 clp1∷ura4 mid1-GFP:kanR ade6-M21X leu1-32 ura4-D18 This study
KGY5626 h− nda3-KM311 cdc15-HA:kanR clp1-MYC:kanR ade6-M21X leu1-32 This study
KGY5627 h− cdc15-GFP:kanR clp1∷ura4 ade6-M21X leu1-32 ura4-D18 This study
KGY5630 h+ nda3-KM311 cdc15-CFP:kanR clp1-YFP:kanR ade6-M21X leu1-32 ura4-D18 This study
KGY5631 h+ nda3-KM311 clp1-GFP:kanR mid1-MYC:ura4 leu1-32 ura4-D18 This study
KGY5667 h+ nda3-KM311 cdc15-CFP:kanR clp1-YFP:kanR mid1∷ura4 ade6-M21X leu1-32 ura4-D18 This study
KGY5812 h+ nda3-KM311 clp1-MYC:kanR mid1∷ura4 ura4-D18 leu1-32 This study
KGY5907 h+ cdc15-GFP:kanR C286S-MYC:kanR ade6-M21X leu1-32 ura4-D18 This study
KGY5924 h− rlc1-GFP:ura4 clp1∷ura4 ade6-M21X leu1-32 ura4-D18 This study
KGY5925 h− rlc1-GFP:ura4 C286S-MYC:kanR ade6-M21X leu1-32 ura4-D18 This study
KGY5944 h− kanR:YFP-rng2 C286S-MYC:kanR ade6-M21X leu1-32 ura4-D18 This study
KGY5946 h− kanR:YFP-rng2 clp1∷ura4 ade6-M21X leu1-32 ura4-D18 This study
KGY5956 h− kanR:GFP-myo2 clp1∷ura4 ade6-M21X leu1-32 ura4-D18 This study
KGY5958 h+ kanR:GFP-myo2 C286S-MYC:kanR ade6-M21X leu1-32 ura4-D18 This study
KGY6039 h− cdc25-22 cdc15-HA:kanR C286S-MYC:kanR ade6-M21X leu1-32 ura4-D18 This study
KGY6040 h− cdc25-22 cdc15-HA:kanR clp1-MYC:kanR ade6-M21X leu1-32 ura4-D18 This study
KGY6462 h− mid1Δ431-481 ade6-M210 leu1-32 ura4-D18 This study
KGY6485 h− mid1Δ431-481 clp1-GFP:kanR ade6-M21X leu1-32 ura4-D18 This study
KGY6486 h− mid1Δ431-481-GFP:kanR ade6-M210 leu1-32 ura4-D18 This study
KGY6487 h− nda3-KM311 mid1Δ431-481 clp1-GFP:kanR ade6-M21X leu1-32 ura4-D18 This study
KGY6549 h− cps1-191 mid1Δ431-481-GFP:kanR ade6-M21X leu1-32 ura4-D18 lys1-151 This study
KGY6597 h− cdc15-140 mid1Δ431-481-GFP:kanR ade6-M21X leu1-32 ura4-D18 This study
KGY6599 h− cdc15-GFP:kanR mid1Δ431-481 ade6-M21X leu1-32 ura4-D18 This study
KGY6605 h− cdc12-112 clp1∷ura4 ade6-M21X leu1-32 ura4-D18 This study
KGY6606 h− cdc12-112 mid1Δ431-481-GFP:kanR ade6-M21X leu1-32 ura4-D18 This study
KGY6607 h− cdc12-112 mid1-GFP:kanR ade6-M21X leu1-32 ura4-D18 This study
KGY6624 h+ cdc3-124 mid1-GFP:kanR ade6-M21X leu1-32 ura4-D18 This study
KGY6626 h+ cdc3-124 mid1Δ431-481-GFP:kanR ade6-M21X leu1-32 ura4-D18 This study
KGY6795 h− mid1Δ431-481 plo1-GFP:kanR ade6-M21X leu1-32 ura4-D18 This study
KGY6796 h− mid1Δ431-481 kanR:GFP-myo2 ade6-M21X leu1-32 ura4-D18 This study
KGY6797 h− mid1Δ431-481 rlc1-GFP:ura4 ade6-M21X leu1-32 ura4-D18 This study
KGY7202 h− mid1Δ431-481:FLAG:kanR kanR:YFP-rng2 ade6-M21X leu1-32 ura4-D18 This study