Figure 2.

Different effect of 15°C temperature block on FP-17 and -22 localization. CV1 cells were microinjected with the cDNA coding for EGFP-17 or -22. After 75 min of incubation at 37°C (a and c), cells were fixed or further incubated at 15°C (b and d) for 3 h before fixation. The cells were immunostained for ERGIC-53, with the use of Alexa 568 secondary antibodies, and analyzed by confocal microscopy. Each column shows the same field visualized for GFP (top) or ERGIC-53 (middle), as well as the merged image with the indicated pseudocolors (bottom). Bar, 10 μm.