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FP-17 and -22 segregation within the ER. Cells were microinjected with cDNAs coding for different couples of FPs, as indicated in the top, and fixed after 75 min at 37°C. The bottom three rows show single-channel and merged images, with the indicated pseudocolors, of enlargements of the boxed areas in the top. Note sheet-like structures in c containing FP-17, from which FP-22 is excluded. Bars: (top) 10 μm; (enlargements) 5 μm.
