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. 2008 Apr 7;181(1):105–118. doi: 10.1083/jcb.200710093

Figure 9.

Figure 9.

State of aggregation of FP-17 and -22 investigated by two different methods. (a) FRAP analysis of the two constructs in the ER. CV1 cells were microinjected with the cDNA coding for EGFP-17, EGFP-22, or VSVG-EGFP. FRAP experiments were performed after 75 min. Values in the ordinate are FI normalized to the prebleach value (see Materials and methods). Data points are means ± SEM for FP-17 (magenta; n = 11), FP-22 (blue; n = 10), and VSVG (orange; n = 6). The table gives the estimated D (in μm2/sec) and Mf values ± SEM. Videos 1 and 2 (available at http://www.jcb.org/cgi/content/full/jcb.200710093/DC1) illustrate typical time-lapse series for FP-17 and -22, respectively. (b) Sucrose gradient analysis of postnuclear supernatants obtained from detergent-lysed CV1 cells 16 h after transfection either with EGFP-17 or -22 (see Materials and methods). Aliquots of the fractions were analyzed by SDS-PAGE followed by Western blotting for ribophorin I (Rb-I) or GFP, as indicated. The arrow and arrowhead indicate the position of cyt c and catalase, run on the gradients as size markers. P, pellet. Numbers on the left of the panels indicate position and Mr (×10−3)of SDS-PAGE size markers.