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SDS-PAGE of purified native as well as recombinant single chain Jacalin. The nJacalin was purified as described earlier (Sastry and Surolia 1986; Sastry et al. 1986; Mahanta et al. 1990, 1992) and the single chain rJacalin was purified as described under Materials and Methods and electrophoresed on 15% SDS-PAGE. (Lane 1) 2 μg of rJacalin, (lane 2) SDS-PAGE standard low-range marker (Bio-Rad). The molecular weights are written in kDa. (Lane 3) 2 μg of purified nJacalin.
