Table 2.
rJacalin | nJacalin | |||||
Quencher | Kq | Fa (%) | Quench (%) | Kq | Fa (%) | Quench (%) |
Acrylamide | 3.49 | 100 | 100 | 3.66 | 100 | 100 |
Kl | 21 | 0.082 | 8 | ND | ND | ND |
CsCl | ND | ND | ND | 29 | 0.05 | 5 |
The quenching measurements were obtained as described in Materials and Methods for nJacalin and rJacalin as well as by following the approaches published earlier (Chipman et al. 1967; Gaikwad et al. 1998). The data are an average of three independent measurements. ND indicates quenching not detectable.