Table 3.
Characteristics of regulated Tg lines
| Regulated Tg line | Genotype | Residual expression, %*†
|
Induction level, % | ||
|---|---|---|---|---|---|
| 2 days | 7 days | 30 days | |||
| Tg(tTA:PrP)1 | tTA/F966:PrP/E6740 | 50 | 15 | 10 | 100–200 |
| Tg(tTA:PrP)2 | tTA/F959:PrP/E7655 | ND | 5 | ND | 50–100 |
| Tg(tTA:PrP)3 | tTA/F959:PrP/E6740 | ND | 15 | ND | 200–400 |
| Tg(tTA:PrP)4 | tTA/F959:PrP/E6550 | ND | 50–100 | ND | 400–800 |
*
Expression levels reported were standardized by using FVB PrPc levels as 100%.
†
Measurements were made after the mice were orally administered doxycycline (2 mg/ml) for 2, 7, or 30 days, as indicated. ND, not determined.