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α-Synuclein filaments grow from annular oligomers at 4°C. (Left) Tapping mode AFM height image of representative α-synuclein particle(s) immediately after Superdex 75 chromatography (A), after incubation at 4°C for 2 d (B,C), 10 d (D–F), and 1 mo (G). (Right) Height cross-section of α-synuclein particle. Scan rate, 1.001 Hz; z-scale, 50.0 nm; cantilever length, 225 μm; tip frequency, 77–94 kHz; set point, 1.5 V.
