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. 2004 Dec;13(12):3285–3297. doi: 10.1110/ps.04928204

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Figure 1. — Urea and thermal unfolding of WT and V91G D-CaM. (A) Urea-induced unfolding of apo-V91G D-CaM (circles) and WT D-CaM (squares) in the presence (closed symbols) and absence (open symbols) of 0.3 mM CaCl₂ monitored using far-UV circular dichroism. (B) Urea-induced unfolding of apo-V91G D-CaM (circles) and WT D-CaM (squares) in the presence (closed symbols) and absence (open symbols) of 0.3 mM CaCl₂ monitored using Tyr-138 fluorescence. (C) Thermal unfolding of apo-V91G D-CaM (circles) and WT D-CaM (squares) in the presence (closed symbols) and absence (open symbols) of 5 mM MgCl₂ monitored using far-UV circular dichroism. (D) Thermal unfolding of apo-V91G D-CaM (circles) and WT D-CaM (squares) monitored using Tyr-138 fluorescence.