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. 2004 Dec;13(12):3161–3171. doi: 10.1110/ps.04980304

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Figure 1. — SDS-PAGE analysis of NADP⁺-dependent G3PDH from A. fulgidus heterologously produced in E. coli. About 5 μg protein were loaded on a 12% gel. After electrophoresis, the gels were stained with Coomassie Blue R250. (Lane 0) molecular mass standard; (lane 1) cell extract; (lane 2) supernatant after heat treatment; (lane 3) G3PDH fraction after chromatography on Resource Q; (lane 4) G3PDH fraction after chromatography on Hydroxyapatite; (lane 5) G3PDH fraction after chromatography on Source 15 Phe.