Table 1.

Purification of NADP⁺-dependent G3PDH from A. fulgidus heterologously produced in E. coli

Step	Protein (mg)	Total activity (U)	Specific activity (U/mg)	Yield (%)
Cell extract	730	1000	1.4	100
Heat treatment	91	980	11	98
Resource Q	58	820	14	82
Hydroxyapatite	23	570	25	57
Source 15 Phe	9.9	300	30	30

The cell extract was prepared from 3.2 g (wet mass) cells. One unit was defined as 1 μmole NADPH oxidation per minute with dihydroxyacetone phosphate at pH 6.6 and 70°C.