Figure 2.

Optimal cross-linker lengths for three different sets of protein models—FGF-2 (solid line), deoxyribonucleoside kinase (dashed line), α-catenin (dotted line)—over potential lengths from 1 Å to 65 Å in 1 Å steps. For each length, discriminable model-pair (directed) coverage was determined at Δ of 3, 6, and 12, respectively. Lengths are indicated (thin dashed vertical lines) for five commercially available cross-linkers: sulfo-DST (6.4 Å), DSG (7.7 Å), DMP (9.2 Å), BS³ (11.4Å), and sulfo-EGS (16.1 Å). The results are tabulated in Table 1.