The impact of structural similarity on disulfide experiments planned by XlinkPlan. The plots show the relationship between coverage percentage C (%), and number of experiments N, at various discriminability levels (solid, Δ = 1; dashed, Δ = 2; dotted, Δ = 4). (A) A set of 21 similar Tfa decoys, all contained within one of the 100 Rosetta clusters, was used for planning. They have a mean pairwise RMSD of 7.9 Å. (B) A random subset of 21 of the 100 final Rosetta decoys was used for planning. They have a mean pairwise RMSD of 13.5 Å.